Studies on Iodinated Compounds. II. High Performance Liquid Chromatographic Studies on the Synthesis and Purification of Monoiodocarnosine

Abstract

Conditions for the synthesis and purification of monoiodocarnosine (MIC) were examined by high performance liquid chromatography (HPLC). When carnosine (β-alanyl-L-histidine) was iodinated to obtain MIC, the maximum yield was attained by the equimolar reaction of carnosine with I₂ in 0.5 N NaOH solution. The synthesized MIC was separated by preparative reversedphase HPLC with H₂O-MeOH (90 : 10) as the eluent and then purified by recrystallization. Chemically pure MIC was obtained in 72% yield in a short time.