A New Excitation Method in Laser Fluorometry and Raman Spectroscopy Using an Optical Fiber-An Application to High Performance Liquid Chromatography

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The use of an optical fiber as a wave guide for a laser beam reduces the undesired scattered light in trace fluorometry and in Raman spectroscopy of microliter-size samples. It also permits effective sample excitation and easy optical alignment. In conjunction with high performance liquid chromatogaphy (HPLC), this excitation method has improved the detection limit by an order of magnitude without any other change in the conventional HPLC fluorometric detection system.