A Potentially Useful Fluorogenic Amine, 4-Amino-7-nitrobenz-2-oxa-1, 3-diazole. An Application as a Substrate for a Microdetermination of Chymotrypsin

Abstract

4-Amino-7-nitrobenz-2-oxa-1, 3-diazole (1b) is a potentially useful key fluorogenic amine. As an example of the application of this amine, 7-(N-tosyl-and N-mesyl-L-phenylalanyl) amino-4-nitrobenz-2-oxa-1, 3-diazole (2) were prepared and shown to be good fluorogenic substrates for the assay of chymotrypsin.