Studies on Peptides. CXXII. N-Succinimidyl-p-(2-nitrovinyl)-benzoate and Its m-Isomer, as Heterobifunctional Conjugating Reagents for Immunoassay

Abstract

N-Succinimidyl-p-and m-(2-nitrovinyl)-benzoates (SNVB) were introduced as heterobifunctional conjugating reagents for immunoassay. These reagents smoothly react with SH-compounds, such as cysteine or mercaptopropionic acid, in acidic media, then with alanine under slightly basic conditions to yield the corresponding benzoylalanine derivatives. On the basis of these model experiments, the feasibility of practical use of these reagents is discussed.