Studies on Peptides. CXVII. Solution Synthesis of the Tetratetracontapeptide Amide Corresponding to the Entire Amino Acid Sequence of Growth Hormone Releasing Factor, Somatocrinin

Abstract

The tetratetracontapeptide corresponding to the entire amino acid sequence of growth hormone releasing peptide derived from human pancreas islet tumor was synthesized by assembling eight peptide fragments in a conventional manner, followed by thioanisole-mediated deprotection with 1 M trifluoromethanesulfonic acid in TFA. Our synthetic peptide significantly stimulated the release of immunoreactive growth hormone in vivo.