Evaluation of ^99mTc-Labeled Amino Acids as Radiopharmaceuticals. VI. N-Pyridoxylidenehydrazine-N', N'-diacetic Acid

Abstract

^<99m>Tc labeled N-pyridoxylidenehydrazine-N', N'-diacetic acid and related hydrazones were evaluated as hepatobiliary imaging agents. Hydrazones used in the study were N-pyridoxylidenehydrazine-N', N'-diacetic acid (PLHzDA), N-(3-hydroxy-4-pyridylmethylene) hydrazine-N', N'-diacetic acid (FHPHzDA), N-(4-pyridylmethylene) hydrazine-N', N'-diacetic acid (INHzDA), and N-pyridoxylidene-N', N'-dimethylhydrazine (PLDMHz). The hydrazones were labeled with ^99mTc by the SnCl₂ method and the ^99mTc labeling was examined by thin-layer chromatography and high-performance liquid chromatography. ^99mTc labeled hydrazones were administered to golden hamsters, and the distribution indicated that clearance occurred through the hepatobiliary system. Scintigraphic studies in rabbits indicated that ^99mTc-labeled PLHzDA and FHPHzDA are useful hepatobiliary radiotracers.