Abstract
Fluorescein monoglucuronide (III), the main metabolite of fluorescein (I), was chemically synthesized by means of the Koenigs-Knorr reaction. Nuclear magnetic resonance (NMR) spectra and mass spectra (MS) showed that the synthesized material was monoglucuronidated fluorescein. The changes in absorption spectra with pH indicated that this material was an "ether" glucuronide. The glucuronoside linkage was concluded to be β, since this material was hydrolyzed by β-glucuronidase. Thus, the synthesized material was confirmed to be fluorescein mono-β-D-glucuronide.
