EFFECTIVE SEPARATION OF STEROL C-24 EPIMERS BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Tohru Kikuchi; Shigetoshi Kadota; Takehiko Shima; Nobuo Ikekawa; Yoshinori Fujimoto

doi:10.1248/cpb.32.3779

Chemical and Pharmaceutical Bulletin

Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363

EFFECTIVE SEPARATION OF STEROL C-24 EPIMERS BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Tohru Kikuchi, Shigetoshi Kadota, Takehiko Shima, Nobuo Ikekawa, Yoshinori Fujimoto

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Keywords: poriferasterol

JOURNAL FREE ACCESS

1984 Volume 32 Issue 9 Pages 3779-3782

DOI https://doi.org/10.1248/cpb.32.3779

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Abstract

Separation of typical C-24 epimers of sterol benzoates was effectively achieved by reversed-phase HPLC under the controlled temperature. It turned out that the retention time of each 24α-epimer is appreciably shorter than that of the corresponding 24β-epimer.

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