1985 年 33 巻 10 号 p. 4152-4159
Ribooligonucleotides (nonamer, decamer and nonadecamer) were synthesized by the solid phase phosphotriester method using 2'-O-tetrahydrofuranyl nucleosides. These oligomers were designed in order to obtain E. coli tRNAMetf deficient in D-loop and D-stem and its analogs. Condensation was performed by using dimer blocks and the dimethoxytrityl group on the 5'-hydroxyl group was removed selectively by 1M ZnBr2 without affecting the 2'-protection.