Anion exchange resins, Duolite A-101D, A-102D, and A-109, were applied to the fluorescent derivatization of estrogens with dansyl chloride (DNS-Cl) for high-performance liquid chromatographic analysis. The derivatization proceeded rapidly and efficiently on passing a mixed solution of estrogens and DNS-Cl in acetone through a small column packed with Duolite A-102D (OH- form) to give the corresponding DNS-estrogens in excellent yields. The procedure using the resin column is of practical value and may be applicable to the derivatization of other analytical samples.
