Abstract
Deoxyribonucleotides containing 2'-deoxyadenosine at the 3'-terminal, i.e., a heptadeoxyribonucleotide, d-GATTCTA, two pentadecadeoxyribonucleotides, d-A15, d-AATGAGAAGCAACGA and a nonadecadeoxyribonucleotide, d-AGAATCTCGTTGCTTCTCA, were synthesized on a polystyrene polymer support by using the phosphotriester method. Dimer blocks (3) were coupled for elongation in the 5'-direction by using 1-mesitylenesulfonyl-3-nitro-1H-1, 2, 4-triazole as the activating reagent. A 3% trichloroacetic acid solution in dichloromethane was used for the detritylation process without causing any detectable depurination. 5'-O-Phosphorylated heptanucleotide and pentadecanucleotide were successfully joined by using deoxyribonucleic acid ligase in the presence of a template.
