Alkaloid Composition and Atropine Esterase Activity in Callus and Differentiated Tissues of Duboisia myoporoides R. BR.

抄録

Alkaloid composition and atropine esterase activity in callus and differentiated tissues from callus and from the original plant of Duboisia myoporoides were determined. Callus and differentiated shoots from callus contained no tropane alkaloids, though they contained very small amounts of anabasine and nicotine. In contrast, differentiated roots from callus contained all the kinds of alkaloids found in the original plant, i.e., atropine, scopolamine, anabasine, nornicotine and nicotine. Neither callus nor differentiated leaves showed atropine esterase activity, but differentiated roots did show atropine esterase activity. These results indicate that the root plays an important role in both tropane alkaloid biosynthesis and degradation, whereas the leaf does not.