Abstract
The effects of Neurotropin (NSP) on lysosomal and microsomal enzyme activities, antioxidant levels and fatty acid composition of microsomal phospholipids in rat liver were studied before and after CCl4 intoxication. The effect on in vitro lipid peroxidation was also determined. NSP posttreatment (60 mg/kg/d for 3 and 7 d after CCl4) did not produce a significant recovery of the enzyme activities, while NSP pretreatment (60 mg/kg/d for 7 d before CCl4) partly prevented the decrease in lysosomal enzyme activities after CCl4 intoxication and increased the level of water-soluble antioxidants as compared with that of CCl4-treated rats. NSP significantly decreased ascorbateinduced lipid peroxidation in vitro, by 20% at the concentration of 1 mg/ml, without affecting the reduced nicotinamide adenine dinucleotide phosphate (NADPH)-induced lipid peroxidation. Further, NSP significantly enhanced, by 20-23%, the protecting effect of inhibitors (CoCl2 and aniline) of lipid peroxidation. Thus, NSP pretreatment appears to have a partial protecting effect against CCl4 intoxication and accompanying lipid peroxidation.
