Fluorimetric Assay for Hydrolytic Activity of Cholesterol Esterase in Rat Tissues
キーワード:
cholesterol
1985 年 33 巻 3 号 p. 1277-1280
詳細
1985 年 33 巻 3 号 p. 1277-1280
A sensitive fluorimetric method for the assay of the hydrolytic activity of cholesterol esterase in enzyme preparations from rat tissues using cholesterol oleate as a substrate is described. The increase in the amount of cholesterol in the enzyme reaction is measured fluorimetrically by using the cholesterol oxidase-peroxidase-3-(p-hydroxyphenyl) propionic acid system. The assay requires only 45-150 μg of protein of preparations from the liver, testis, adipose tissue of the mesentery and adrenal gland.
