Identification of Steroidal Compounds as Acetates in a Lysate of "Depressor-I", an Antihypertensive Phospholipid Occurring in Acetone-Soluble Fraction of Bovine Brain

Abstract

The alkaline hydrolysate of "Depressor-I", an antihypertensive phospholipid occurring in the acetone-soluble fraction of bovine brain, was [³H] acetylated and applied to an alumina column. Various [³H] acetylated compounds were eluted. Among the major fractions, the eluates with hexane and hexane-benzene (9 : 1, 7 : 3 and 5 : 5, v/v) mixtures contained labile compounds which decomposed on standing for one month. Gas chromatography-mass spectrometry was carried out with the corresponding sample obtained in a "cold"experiment, and diosgenin acetate, a sapogenin, was detected besides cholesteryl acetate. A new class of phospholipid containing steroidal components is proposed.