Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
New Fluorogenic Substrates for Subtilisin
YUICHI KANAOKA, TETSUO TAKAHASHI, HITOSHI NAKAYAMA, KAZUTAKA TANIZAWA
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Keywords: kinetic study
JOURNAL FREE ACCESS

1985 Volume 33 Issue 4 Pages 1721-1724

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Abstract
Two 7-amino-4-methylcoumarinamide derivatives of tripeptides were synthesized. They were shown to be potentially useful substrates for fluorometric microdetermination of subtilisins BPN'and Carlsberg. The kinetic characteristics of the hydrolyses of these substrates by the enzymes were investigated.
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© The Pharmaceutical Society of Japan
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