Abstract
A deoxyribonucleic acid (DNA) duplex with a chain length of 46 which contain binding sites for catabolite gene activator protein and ribonucleic acid (RNA) polymerase was synthesized by the solid-phase phosphotriester method, involving condensation of tetramer or pentamer blocks. Eleven oligonucleotide blocks were used to elongate each chain, beginning from polystyrene-linked N-protected 3'-succinyldeoxynucleoside. The estimated overall yields of upper and lower chains were 18% and 17%, respectively. The products were purified by reversed phase chromatography and characterized by sequence analysis using the Maxam-Gilbert method.
