Abstract
The chemical modification of adenine moieties of mouse 5S ribosomal ribonucleic acid (RNA) was carried out with monoperphthalic acid in order to define the secondary structure of mouse 5S ribosomal RNA. The analysis of the modified 5S ribosomal RNA showed that the adenine residues at positions 11, 13 (or/and 16), 22, 23, 49, 50, 54, 55, 77, 83, 88, 90, 100, 101 and 103 were modified to adenine N-oxide residues. This result is consistent with a secondary structure of mouse 5S ribosomal RNA consisting of 5 loops and 5 helices with three bulge structures involving cytosine and adenine residues.
