Urea Sensor Based on an Ion Sensitive Field Effect Transistor. II. Effects of Buffer Concentration and pH on the Potentiometric Response
キーワード:
immobilized enzyme
1985 年 33 巻 6 号 p. 2556-2559
詳細
1985 年 33 巻 6 号 p. 2556-2559
The effects of pH and concentration of working buffer on the potentiometric response of a urea sensor based on an ion-sensitive field effect transistor (ISFET) were examined. The sensor showed a higher response in slightly acidic buffer solution (pH 6.0) than in buffer solutions of pH 7.1 and 8.0. The pH dependence of the response of the sensor can be explained in terms of the activity of urease immobilized on the ISFET. The buffer concentration also affected the response. Higher concentration buffers (20 and 50 mM) strongly disturbed the response due to the higher buffer capacity.
