Isolation of an Insect-Antifeedant, Phloretin 4'-O-β-D-Glucopyranoside, by Rotation Locular Counter-Current Chromatography and Determination of Its Preferred Conformation in Solution by Nuclear Magnetic Resonance Analysis

Abstract

A bitter insect-antifeedant dihydrochalcone, phloretin 4'-O-β-D-glucopyranoside, was isolated from fresh apple leaves by using rotation locular counter-current chromatography (RLCC), and the preferred conformation in solution was determined by means of proton (¹H-) and carbon-13 (¹³C-) nuclear magnetic resonance (NMR) experiments.