SYNTHESIS OF A 27-RESIDUE PEPTIDE CORRESPONDING TO THE ENTIRE AMINO ACID SEQUENCE OF HUMAN GASTRIN-RELEASING PEPTIDE (hGRP)

Abstract

Human GRP (gastrin-releasing peptide) was synthesized in a conventional solution method by assembling six peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. Before the deprotection, the two Met sulfoxides employed were reduced by phenylthiotrimethylsilane. The synthetic peptide raised the immunoreactive gastrin level in rat plasma like synthetic porcine GRP.