Spectrophotometric Determination of Papaverine Hydrochloride by Using a Membrane Filter Preconcentration Technique with 2, 4, 5, 7-Tetrachlorofluorerscein and Palladium(II)

Abstract

A simple, rapid and highly sensitive spectrophotometric determination of paraverine hydrochloride (Papav. HCl) by using a preconcentration technique based on membrane filtration of the 2, 4, 5, 7-tatrachlorofluorescein (T.Cl.fl.)-palladium(II)-Papav. HCl ternary complex was developed. A linear calibration graph was obtained in the concentration range of 0-20 μg of Papav. HCl in 5 ml of dimethyl sulfoxide (DMSO). The apparent molar absorptivity for Papav. HCl was 8.0×10⁴ dm³ mol^-1 cm^-1 at 540 nm, and was more than twice that measured in an aqueous medium.