A NEW CONVENIENT METHOD FOR MICRODETERMINATION OF HYDROXYPROLINE BY FLOW INJECTION ANALYSIS

Abstract

A more rapid and convenient microdetermination of hydroxyproline(Hyp) was established by modification of the conventional KISO method using flow injection analysis. This new flow method is based on the color change reaction between pyrole, the oxidation and decarboxylation product of Hyp, and Ehrlich's reagent. A good linear relationship was obtained between the concentration of Hyp (from 0 to 80 μg/ml) and absorbance.