Application of Borate Ion-Exchange Mode High-Performance Liquid Chromatography to Separation of Glycosides : Saponins of Ginseng, Sapindus mukurossi GAERTN. and Anemone rivularis BUCH.-HAM.

HIROYUKI YAMAGUCHI; HIROMICHI MATSUURA; RYOUJI KASAI; KENJI MIZUTANI; HIROKO FUJINO(KIMATA); KAZUHIRO OHTANI; TORU FUWA; OSAMU TANAKA

doi:10.1248/cpb.34.2859

HIROYUKI YAMAGUCHI, HIROMICHI MATSUURA, RYOUJI KASAI, KENJI MIZUTANI, HIROKO FUJINO(KIMATA), KAZUHIRO OHTANI, TORU FUWA, OSAMU TANAKA

Author information

HIROYUKI YAMAGUCHI
HIROMICHI MATSUURA
RYOUJI KASAI
KENJI MIZUTANI
HIROKO FUJINO(KIMATA)
KAZUHIRO OHTANI
TORU FUWA
OSAMU TANAKA
Author's Organization：
Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd.,
Central Research Laboratories, Wakunaga Pharamaceutical Co., Ltd.,
Japan, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
Japan, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
Japan, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
Japan, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
Central Research Laboratories, Wakunaga Pharamaceutical Co., Ltd.,
Japan, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine

Keywords: steviol glycoside

JOURNAL FREE ACCESS

1986 Volume 34 Issue 7 Pages 2859-2867

DOI https://doi.org/10.1248/cpb.34.2859

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Abstract

High-performance liquid chromatography (HPLC) of borate complexes on a basic ion-exchange column was investigated for the separation of saponins of Ginseng, Sapindus mukurossi and Anemone rivularis, as well as other synthetic glycosides including steviol glycosides. It was found that HPCL in this mode is effective for analysis and preparative separation of a variety of glycosides, especially isomeric glycosides, containing xylopyranosyl, arabinofuranosyl or arabinopyranosyl units.

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