Electric Microassays of Glucose, Uric Acid and Cholesterol Using Peroxidase Adsorbed on a Carbon Electrode

Abstract

Highly sensitive electrochemical mathods for the assays of glucose, uric acid and total cholesterol in serum were established by using peroxidase adsorbed on a carbon electrode.Glucose, uric acid and cholesterol were oxidized by glucose oxidase uricase and cholesterol oxidae, respectively, to give a hydrogen peroxide, which was measured by means of a hydrogen peroxide sensor using peroxidase adsorbed on a carbon electrode. Each method required 5 μl of serum, and the detection limits of glucose, uric acid and total cholesterol were 0.10 μg (0.555 nmol), 0.05 μg (0.295 nmol), and 0.25 μg (0.650 nmol) per assay tube, respectively.