9-ANTHRYLDIAZOMETHANE-HPLC METHOD FOR DETECTION OF PROSTGLANDINS AND THROMBOXANE : AN APPLICATION TO THE MEASUREMENT OF THE PRODUCTS OF STIMULATED RABBIT PLATELETS

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The cyclooxygenase products of rabbit platelets stimulatd with arachidonic acid were measured by HPLC. Prostaglandins and thromboxane were extracted, derivatized with 9-anthryldiazomethane, and then loaded on an ODS-column. The fluorescence of the eluate was monitored. Thromboxane B₂ was a major product, which returned to the control level in the presence of indomethacin or the thromboxane synthetase inhibitor OKY-046. Pretreatment with OKY-046 shifted the cyclooxygenase products to increased levels of PGE₂, PGD₂ and PGF_2α.