Photoaffinity Labeling of a δ-Receptor Component of NG 108-15 Cells with a New Enkephalin Analog

KUNIO OKUMURA; YASUMARU HATANAKA; HITOSHI NAKAYAMA; YUICHI KANAOKA

doi:10.1248/cpb.35.256

KUNIO OKUMURA, YASUMARU HATANAKA, HITOSHI NAKAYAMA, YUICHI KANAOKA

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Keywords: photoaffinity labeling, δ-opioid receptor, NG 108-15 cell, enkephalin analog, polypeptide

JOURNAL FREE ACCESS

1987 Volume 35 Issue 1 Pages 256-261

DOI https://doi.org/10.1248/cpb.35.256

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Abstract

A new leucine-enkephalin analog containing p-azidophenylalanine was synthesized. It binds to the δ-opioid receptors of NG 108-15 cells with high affinity. Iodination of the compound decreased the affinity by 5 times, but the iodinated derivative still retains significant binding activity to the cells. Photochemical reactions revealed that the derivative predominantly labeled a 58 kilodaltons (kDa) polypeptide in the cells (possibly a δ-receptor component)

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