Synthesis of a 37-Residue Peptide Amide Corresponding to the Entire Amino Acid Sequence of α-Form of Rat Calcitonin Gene-Related Peptide (α-rCGRP)

Abstract

The heptatriacontapeptide amide corresponding to the entire amino acid sequence of α-form of rat calcitonin gene-related peptide (α-rCGRP) was synthesized by the conventional solution method. All protecting groups employed were removed by treatment with 1 M trifluoromethanesulfonic acid-thioanisole-trifluoroacetic acid, and the deprotected peptide was subjected to airoxidation to form the intramolecular disulfide bond. After purification by gel-filtration on Sephadex G-50, followed by reversed-phase high performance liquid chromatography, a highly purified sample of synthetic α-rCGRP was obtained. In terms of suppression of bone ⁴⁵Ca-release stimulated by synthetic human parathyroid hormone (1-34), synthetic α-rCGRP was as active as synthetic human CGRP.