Direct Liquid Chromatographic Resolution of Racemic Compounds. Use of Ovomucoid as a Column Ligand

TOSHINOBU MIWA; MASAKI ICHIKAWA; MASANORI TSUNO; TEIICHI HATTORI; TAKESHI MIYAKAWA; MASANORI KAYANO; YASUO MIYAKE

doi:10.1248/cpb.35.682

TOSHINOBU MIWA, MASAKI ICHIKAWA, MASANORI TSUNO, TEIICHI HATTORI, TAKESHI MIYAKAWA, MASANORI KAYANO, YASUO MIYAKE

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Keywords: chiral resolution, affinity column, HPLC, enantiomer, ovomucoid

JOURNAL FREE ACCESS

1987 Volume 35 Issue 2 Pages 682-686

DOI https://doi.org/10.1248/cpb.35.682

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Abstract

A new chiral recognition column, with conjugated ovomucoid as the ligand, was developed. This column may be employed for the chiral resolution of acids as well as amines without derivatization. The retention time, capacity factor and resolution factor were dependent on pH, buffer strength and 2-propanol concentration of the mobile phase. For chlorpheniramine, a resolution factor of 1.5 was obtained.

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