Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
Glycyrrhizin β-D-Glucuronidase of Eubacterium sp. from Human Intestinal Flora
TAIKO AKAOTERUAKI AKAOKYOICHI KOBASHI
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1987 Volume 35 Issue 2 Pages 705-710

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Abstract

A bacterial strain capable of hydrolyzing glycyrrhizin (GL) to glycyrrhetic acid (GA) was isolated from human feces. This bacterium was identified as Eubacterium sp. The GL-hydrolyzing activity increased in parallel with the growth of this bacterium, which also produced β-D-glucuronidase (EC 3.2.1.31) acting on β-D-glucuronides of phenolic compounds such as phenolphthalein mono-β-D-glucuronide. GL-hydrolyzing activity was recovered in the supernatant fraction after disruption of this bacterium with a French press and was partially purified by means of ammonium sulfate fractionation, and Sephadex G-200 and octyl-Sepharose column chromatographies.
GL-hydrolyzing enzyme was separated from the β-D-glucuronidase which hydrolyzes β-D-glucuronides of phenolic compounds by octyl-Sepharose column chromatography, indicating that the GL-hydrolyzing enzyme is a novel type of β-D-glucuronidase.

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© The Pharmaceutical Society of Japan
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