Determination of Ticlopidine in Rabbit Plasma by High-Performance Liquid Chromatography

Abstract

A method for determination of ticlopidine in plasma by high-performance liquid chromatography was established. The plasma samples (0.2 ml) were extracted with hexane. After evaporation, the hexane extracts were redissolved in the mobile phase containing phenobarbital as an internal standard, and an appropriate volume was injected onto a column of Cosmosil 5C8 (150 × 4 mm, i.d.); mobile phase, acetonitrile-10 mM phosphate buffer of pH 4.0 (30 : 70 v/v); flow rate, 1.0 ml/min; spectrophotometric detection at 230 nm. Ticlopidine and the internal standard were separated from interfering plasma components by this method. The peak height ratio of ticlopidine to the internal standard was proportional to the ticlopidine concentration in the range from 0.1 to 2.0 μg/ml.