Synthesis of Head Activator (HA) -Related Peptides and Development of HA-Radioimmunoassay

NAOKI SAKURA; MIWAKO NISHIJIMA; YOSHIKI UCHIDA; TETSUHIRO TANI; TADASHI HASHIMOTO

doi:10.1248/cpb.35.2327

NAOKI SAKURA, MIWAKO NISHIJIMA, YOSHIKI UCHIDA, TETSUHIRO TANI, TADASHI HASHIMOTO

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Keywords: head activator, synthesis, antibody preparation, ¹²⁵I-Tyr¹¹-head activator, radioimmunoassay, specificity, droplet counter current chromatography, HPLC

JOURNAL FREE ACCESS

1987 Volume 35 Issue 6 Pages 2327-2333

DOI https://doi.org/10.1248/cpb.35.2327

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Abstract

Synthesis of peptides related to hydra head activator (HA; pGlu-Pro-Pro-Gly-Gly-Ser-Lys-Val-Ile-Leu-Phe-OH) was performed by the solution method. The analogs synthesized were Tyr¹¹-HA, des-Phe¹¹-HA, Arg¹-HA and Phe⁵-HA. Antisera were prepared by immunizing three rabbits with the synthetic HA. Two anti-HA-antisera with sufficient titer were obtained. An HA-radioimmunoassay system, developed by using an antiserum (ASH-04) and ¹²⁵I-Tyr¹¹-HA as a tracer, was found to be sensitive and specific to HA.

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