Abstract
The hexacosapeptide corresponding to the entire amino acid sequence of human α2-plasmin inhibitor fragment T-11 was synthesized by a conventional solution method. Three newly synthesized fragments were combined successively with the protected C-terminal hexadecapeptide ester previously obtained by using the dicyclohexylcarbodiimide-N-hydroxybenzotriazole and azide procedures to afford the protected hexacosapeptide ester. The trifluoromethanesulfonic acid-thioanisole-trifluoroacetic acid procedure was employed to remove all protecting groups of the protected peptide ester at the final stage. The dissociation constant for the interaction between the synthetic α2-plasmin inhibitor fragment T-11 and plasmin was equal to that of the native T-11 from human α2-plasmin inhibitor.
