A tetracontapeptide corresponding to the entire amino acid sequence of a frog skin peptide, sauvagine, was synthesized by assembling eight peptide fragments of established purity followed by deprotection with 1 M trimethylsilyl trifluoromethanesulfonate-thioanisole in trifluoroacetic acid. The synthetic peptide stimulated secretion of immunoreactive corticotropin from rat pituitary cells and its potency was estimated to be equivalent to that of synthetic ovine corticotropin releasing factor. Synthetic sauvagine displayed long-lasting hypotensive action in rats.
