Enzymatic Analysis of Cyanogenic Glycosides. II. : A Simple Method by Using a Microdiffusional Apparatus
1988 年 36 巻 1 号 p. 249-253
詳細
1988 年 36 巻 1 号 p. 249-253
A simple method for the determination of cyanogenic glycosides by using a Conway's microdiffusional apparatus was investigated. The hydrolysis of cyanogenic glycosides by enzymes and the isolation of cyanide liberated were simultaneously performed in the same apparatus. The liberated hydrogen cyanide was determined by Epstein's method using pyridine-pyrazolon reagent. By this method, the recoveries of linamarin and amygdalin were over 90% and the coefficients of variation were 0.2 to 3.6% for both glycosides in the range of 0.5-400 μg of cyanide. This method was applied to the determination of cyanogenic glycosides in pastes of butter beans and apricot kernels and the results were compared with those obtained by the steam distillation method.
