Abstract
Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ISFET sensor, which can measure concentrations of N-benzoyl-L-arginine ethyl ester in the range from 5×10-4 to 5×10-2M. The other is an α-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5×10-4-5×10-3M. The response of the sensors was affected markedly by pH and the concentration of the working buffer.
