Abstract
By combining the theophylline inhibition assay for placential isoenzyme analysis with the lectin precipitation procedure for bone isoenzyme detection, a new more complete method for alkaline phosphatase isoenzyme analysis was devised. Moreover, the method employs common clinical laboratory instruments and is easy to perform.After an examination of the method and its verification by electrophoretic methods with commercial control samples and clinical samples, a careful study was performed to determine the normal ranges. These results were then used as guidelines for placing patients in clinical categories. Verification of these categories by other clinical data on the patients confirmed the validity of the method.
