Preparation and Characterization of Monoclonal Antibodies Specific for Ribonuclese Inhibitor from Rat Liver

Abstract

Monoclonal antibodies specific for ribonuclease inhibitor (RNasin) were obtained by the method of production of hybridomas from spleen cells and myeloma cells. The specificity of monoclonal antibodies obtained by cloning was investigated by Western blot analysis. The addition of ribonuclease (RNase) A to RNasin resulted in the inhibition of antibody binding to the antigen. However, the addition of RNase A to the antigen-antibody complex did not cause dissociation of the complex.