抄録
N, N'-Diphenylethylenediamine (DPED) was found to be a useful hydrogen donor for the determination of hydrogen peroxide in the presence of peroxidase. Pigments formed by this reaction had a peak absorbance at 450nm and had relatively high absorptivity; the measurable range of hydrogen peroxide was between 0.15 and 10 μM. The intra-assay and inter-assay precisions (coefficients of variation) of hydrogen peroxide determination were below 0.99 and 1.86%, respectively. The recovery was 97.2-102.8% (n=10).
