Abstract
The utility of the absorption promoters, sodium glycocholate (GC-Na), ethylenediamine dihydrochloride (EDTA-2Na), sodium caprylate (Cap-Na) and sodium salicylate (Sal-Na), in the intranasal administration of human fibroblast interferon-β (HuIFN-β) in rabbits was investigated. The optimal amount of added EDTA-2Na, Cap-Na and Sal-Na with respect to HuIFN-β was examined for nasal absorption in the powder dosage form. Formulations of HuIFN-β with GC-Na showed greatly enhanced intranasal HuIFN-β absorption, as compared to the other absorption promoters. The results of a stability study on HuIFN-β in homogenates of nasal mucosa suggested that GC-Na behaved as a hydrolysis inhibitor in the nasal mucosa and maintained the activity of HuIFN-β.
