UNEXPECTED LOSS OF IMMUNOREACTIVITY OF A GLUCOSE OXIDASE-LABELED ANTIGEN IN A STEROID ENZYME IMMUNOASSAY SYSTEM

Hiroshi HOSODA; Misuzu ITOH; Reiko TSUKAMOTO; Toshio NAMBARA

doi:10.1248/cpb.37.859

著者情報

キーワード: enzyme immunoassay, enzyme labeling, 11-deoxycortisol, glucose oxidase, glucose oxdase-steroid conjugate, monoclonal antibody

ジャーナルフリー

1989 年 37 巻 3 号 p. 859-860

DOI https://doi.org/10.1248/cpb.37.859

詳細

抄録

Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.

責任著者(Corresponding author)

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