Studies on Sialic Acids. XIII. : Isolation of 3-Deoxy-D-glycero-D-galacto-2-nonulopyranosonic Acid (KDN) from Chum Salmon, Oncorhynchus keta

Mitsunobu NAKAMURA; Kimio FURUHATA; Kiyoko YAMAZAKI; Haruo OGURA; Hisao KAMIYA; Hitoshi IDA

doi:10.1248/cpb.37.2204

Mitsunobu NAKAMURA, Kimio FURUHATA, Kiyoko YAMAZAKI, Haruo OGURA, Hisao KAMIYA, Hitoshi IDA

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Keywords: sialic acid, 3-deoxy-D-glycero-D-galacto-2-nonulopyranosonic acid, KDN, HPLC, isolation, chum salmon

JOURNAL FREE ACCESS

1989 Volume 37 Issue 8 Pages 2204-2206

DOI https://doi.org/10.1248/cpb.37.2204

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Abstract

A method for analysis of 3-deoxy-D-glycero-D-galacto-2-nonulopyranosonic acid (KDN) by high-performance liquid chromatography (HPLC) with a strongly basic anion-exchange resin was developed. The method was applied to detect free KDN in water used to rinse of fertilized eggs of chum salmon, Oncorhynchus keta. Furthermore, KDN was isolated from the water as a fully protected derivative.

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