Synthesis of a Thymosin β4-Like Peptide, Deacetyl-thymosin βXen4, and Its Restorative Effect on Depressed Lymphocyte Blastogenic Response to Phytohemagglutinin (PHA) in Uremic Patients

Takashi ABIKO; Hiroshi SEKINO

doi:10.1248/cpb.37.2467

Synthesis of a Thymosin β₄-Like Peptide, Deacetyl-thymosin β^Xen₄, and Its Restorative Effect on Depressed Lymphocyte Blastogenic Response to Phytohemagglutinin (PHA) in Uremic Patients

安孫子敬, 関野宏

著者情報

キーワード: deacetyl-thymosin β^Xen₄ synthesis, trifluoromethanesulfonic acid deprotection, dithiothreitol reduction, uremic patient, impaired T-lymphocyte blastogenic response, restoring effect, fluorometric blast-formation test

ジャーナルフリー

1989 年 37 巻 9 号 p. 2467-2471

DOI https://doi.org/10.1248/cpb.37.2467

詳細

抄録

An analog of thymosin β^Xen₄ isolated from ooxytes of Xenopus laevis, deacetyl-thymosin β^Xen₄, was synthesized by assembling 6 peptide fragments, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1 : 1) in trifluoroacetic acid in the presence of dimethylselenium. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain. The synthetic tritetracontapeptide was found to have a restoring effect on the impaired blastogenic response of T-lymphocytes isolated from uremic patients.

責任著者(Corresponding author)

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