Production of Highly Specific Anti-testosterone Antiserum by an Immunotolerance Method

Yoshinori MIYAKE; Minoru SHIGETOSHI; Masaharu KOJIMA

doi:10.1248/cpb.38.150

Yoshinori MIYAKE, Minoru SHIGETOSHI, Masaharu KOJIMA

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Keywords: testosterone radioimmunoassay, immunotolerance method, 15α-carboxymethyltestosterone, 15β-carboxymethyltestosterone, anti-testosterone antiserum, cross-reactivity

JOURNAL FREE ACCESS

1990 Volume 38 Issue 1 Pages 150-152

DOI https://doi.org/10.1248/cpb.38.150

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Abstract

Anti-testosterone antisera were produced by pretreatment of rabbits with 15α-carboxymethyl-5α-dihydrotestosterone linked to a copolymer of D-glutamic acid and D-lysine (D-GL) before immunization with the bovine serum albumin conjugate of 15α- and 15β-carboxymethyltestosterone. The specificity for 5α-dihydrotestosterone of the anti-testosterone antisera was considerably improved.

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