Synthesis of Human Splenin (hSP) and Examination of Its Immunological Effects on the Impaired T-and B-Lymphocytes in Uremic Patients

Abstract

Human splenin (hSP) was synthesized by assembling eight peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratios, 1 : 1) in trifluoroacetic acid in the presence of m-cresol and dimethylselenium. Finally, the depretected peptide was insubated with dithiothreitol to reduce sulfoxide on the methionine side chain. Incubation of peripheral lymphocytes isolated from uremic patients with the synthetic hSP showed an enhancing effect on the reduced B-lymphocytes, but had no restoring effect on the impaired blastogenic response of T-lymphocytes.