Micro-Enzyme Immunoassay of Vasoactive Untestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk

Masaharu TAKEYAMA; Katsumi WAKAYAMA; Fusako TAKAYAMA; Kazuhiro KONDO; Nobutaka FUJII; Haruaki YAJIMA

doi:10.1248/cpb.38.960

Masaharu TAKEYAMA, Katsumi WAKAYAMA, Fusako TAKAYAMA, Kazuhiro KONDO, Nobutaka FUJII, Haruaki YAJIMA

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Keywords: vasoactive intestinal polypeptide (VIP)-like immunoreactive substance, bovine foremilk, VIP(11-28)-linked β-D-galactosidase, 4-methylumbelliferyl β-D-galactopyranoside, highly sensitive enzyme immunoassay

JOURNAL FREE ACCESS

1990 Volume 38 Issue 4 Pages 960-962

DOI https://doi.org/10.1248/cpb.38.960

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Abstract

A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal(C-terminal) fragment (residue 11-28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was 0.1 fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100 pmol/l, which was more then eightfold higher than that in normal milk.

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