1990 年 38 巻 5 号 p. 1323-1325
A new type of chemiluminogenic substrate for N-acetyl-β-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-β-D-glucosaminide, was prepared by incorporating an enzyme-removable substituent, N-acetyl-D-glucosaminide group, into the hydrazide moiety of luminol. This substrate releases luminol upon enzymatic hydrolysis. The enzyme was detected chemiluminescently using this substrate.