1, 2-Diarylethylenediamines as Pre-column Fluorescence Derivatization Reagents in High-Performance Liquid Chromatographic Determination of Catecholamines in Urine and Plasma

Abstract

meso- and dl-1, 2-diarylethylenediamines (14 species) were evaluated for pre-column fluorescence derivatization reagents in the high-performance liquid chromatographic determination of catecholamines (norepinephrine, epinephrine and dopamine) in human urine and plasma. Of the compounds, meso-1, 2-bis(4-methoxyphenyl)ethylenediamine was most preferable for all the catecholamines in terms of sensitivity and selectivity. The detection limit for each catecholamine is approximately 0.5 fmol in a 50-μl injection volume.