Synthesis of Rat Parathymosin α Fragment 1-28 and Examination of Its Inhibitory Activity towards the Restoring Activity of Thymosin α₁ on the Impaired T-Lymphocytes of Uremic Patients

Abstract

A fragment corresponding to N-terminal octaeicosapeptide of rat parathymosin α was synthesized by assembling 5 peptide fragments, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio 1 : 1) in trifluoroacetic acid in the presence of dimethylselenium. Incubation of impaired T-lymphocytes isolated from uremic patients with the synthetic parathymosin α fragment 1-28 showed no immunological restoring effect, but when it was administered together with thymosin α₁ it appeared to suppress the restoring effect of the thymosin α₁ on the impaired T-lymphocytes of uremic patients.