A New Monitoring System of Cultured Myocardial Cell Motion : Effect of Pilose Antler Extract and Cardioactive Agents on Spontaneous Beating of Myocardial Cell Sheets

Abstract

Effects of various cardioactive agents and a water extract of the pilose antler of Cervus nippon var. mantchuricus on periodic beating of cultured myocardial cell sheets were examined by using an image analyzing system. Norepinephrine increased the beating rate and the beating amplitude, whereas digoxin and forskolin enlarged only the beating amplitude. Verapamil and propranolol decreased both the beating rate and the beating amplitude. The water extract of the pilose antler showed no remarkable effects in a standard medium (2.1 mM Ca²⁺). However, it significantly increased the beating amplitude when the beating was suppressed by replacement with a low calcium medium (0.5 mM Ca²⁺). A similar effect was found for 70% ethanol-soluble and -insoluble fractions of the extract.